Application

Western blot: 1:1000 - 1:2000 (THP-1 whole cell lysate can be used as a positive control)

Optimal working dilutions must be determined by end user.

Anti-C-C Chemokine Receptor 5 Antibody, N-terminus is an antibody against C-C Chemokine Receptor 5 for use in WB.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HIV and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G-protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1,2). Among them, CCR5 is a principle coreceptor for macrophage- and dual-tropic HIV-1 strains fusion of entry of human white blood cells (3,4). The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Immunogen

Synthetic peptide from the N-terminal domain of human CCR5.

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. 100 µg purified IgG in 100 µL of PBS containing 0.2% sodium azide.

Format: Purified

Specificity

Specific for human C-C Chemokine Receptor 5 (CCR5) at the N-terminus.

Storage and Stability

Store at 2-8°C for up to 12 months.